BiologyBotany QuestionsBiotechnology: Principles And Processes Questions for CBSE Class 11th

Biotechnology: Principles And Processes Questions for CBSE Class 11th

A special type of DNA polymerase used during PCR is:

Transgenic plants are the ones

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    The bacterial cells are made competent by incubating the bacterial cells in:

    Taq polymerase enzyme is used in

    In recombinant DNA technique the term vector refers to

    GAATTC is the r ecognition site for which of the following restriction endonuclease?

    In which one of the following methods rDNA is incorporated into host cells?

    What we call the extra chromosomal circular DNA molecules of bacteria used to carry genes of interest into the host cell?

    An enzyme catalyzing the removal of nucleotide from the ends of DNA is

    Which of the following is not a feature of the plasmids?

    DNA fragments are

    Which gene is inserted as marker in screening recombinant transformants from non-recombinant transformants in a plasmid vector?

    Choose the correct statement.

    Identify correct statement in context to gel electrophoresis.

    Following statements describe the characteristics of the cloning vectors. Identify the incorrect statement.

    In recombinant DNA technology, chilled ethanol is used for-

    Which of the following is a thermostable enzyme?

    DNA elution is related to one of the following methods or technique?

    Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme ?

    There is a restriction endonuclease called Eco RI .What does ‘ co ’ part in it stand for ?

    The DNA fragments separated on an agarose gel can be visualised after staining with

    Match Column I with Column II Column I Column II a. Stanley Cohen and Herbert Boyer i. Developed technique of DNA Fingerprinting b. Alec Jeffreys ii. Transplanted genes from one living organism to another c. Kary Mullis iii. Invented Polymerase Chain Reaction

    Read the following statements about agarose gel electrophoresis and choose the incorrect option.

    Given diagram showing a typical agarose gel electrophoresis showing migration of DNA gragments. The undigested DNA fragment will be present in the lane.

    The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme.

    Transgenic plants are the ones:

    During the process of isolation of DNA, chilled ethanol is added to

    The introduction of T-DNA into plants involves

    What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

    Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

    The correct order of steps in Polymerase Chain Reaction (PCR) is

    The specific palindromic sequence which is recognized by EcoRI is :

    The extent of digestion of genomic DNA of donor organism is tested with the help of the following technique.

    In recombinant DNA technology, elimination of non- transformant and selective growth of transformants is possible due to

    Due to insertion of desired gene within selectable marker gene of vector, selectable marker gene become inactive or loose their function. This is called:

    Two bacteria found to be very useful in genetic engineering experiments are

    Read the following statements and choose the correct option. Statement I : Agrobacterium tumefaciens is the causative agent of crown gall disease in monocots. Statement II : Agrobacterium tumefaciens causes plant tumour when a specific part of DNA from the Ti plasmid gets integrated with the plant chromosome.

    List – I List – II A) Lysozyme i) Makes the DNA visible as bright orange object when exposed to UV radiation B) Chilled ethanol ii) Huge vessel used to obtain recombinant protein C) Bioreactor iii) Precipitates the genomic DNA from other degraded components of cell. D) Ethidium bromide iv) Hydrolyses peptidoglycan

    Match the following and choose the correct combination from the options given below. Column – I Column – II A. Escherichia coli I. nif gene B. Bacillus thuringiensis II. Interferon C. Rhizobium meliloti III. Bt toxin D. Agrobacterium tumefaciens IV. Vector A B C D 1. II I IV III 2. II I III IV 3. II III I IV 4. IV I II III

    Who is regarded as ‘Father of Genetic engineering’ ?

    Polymerase chain reaction is most useful in

    Which of the following bacteria has found extensive use in genetic engineering work in plants ?

    Large scale production of biotechnological products involves use of

    In recombinant DNA technology, a plasmid vector must be cleaved by

    “Lal Bahadur Shastri centre for advanced Research in Biotechnology” is situated at

    The first artificial cloning vector was

    Restriction endonuclease cuts

    Restriction enzymes are isolated chiefly from

    The technique in which a foreign DNA precipitated on the surface of tungsten or gold particles and shot into the target cells is known as

    Which of the following key factors makes the plasmid, the vector in genetic engineering ?

    T m represents

    The vectorless gene transfer includes a. particle gun b. microinjection c. electroporation

    First step in genetic engineering is

    Which of the following is a plasmid ?

    Which of the following is correctly matched ?

    Biolistics (gene-gun) is suitable for

    DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

    Restriction enzyme Eco Rl cuts the DNA between bases G and A only when the sequence in DNA is

    Which vector can clone only a small fragment of DNA?

    A gene whose expression helps to identify transformed cell is known as

    A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

    PCR proceeds in three distinct steps governed by temperature, they are in order of

    The DNA molecule to which the gene of interest is integrated for cloning is called

    The process of separation and purification of expressed protein before marketing is called

    The correct order of steps in Polymerase Chain Reaction (PCR) is

    In the process of rDNA technology, the rDNA is incorporated into host bacterial cell by a phenomenon known as

    Eco R I recognises a base sequence which has

    A cloning vector has tetracycline and ampicillin resistant gene. A foreign gene was inserted in the ampicillin resistant gene. The recombinant would survive in a medium containing:

    Arrange the three given basic steps of genetically modifying an organism in correct sequence. A. Introduction of the identified DNA into the host. B. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny. C. Identification of DNA with desirable genes.

    Choose the DNA sequence that is most likely to be cleaved by restriction endonucleases.

    Find the correct match.

    If alien DNA is introduced using Pst I in pBR322 then the recombinant transformant can grow on :

    Which amongst the following character is not desirable in a cloning vector?

    Which amongst the following statement is true about restriction endonucleases?

    Arrange the following steps for making the bacterial cell competent to take up DNA, in chronological order. I. Incubation of cells with recombinant DNA on ice. II. Incubation of cells with recombinant DNA at 42 degree Celsius III. Treating cells with a specific concentration of a divalent such as calcium

    If the gene of interest is introduced in a tetracycline – resistant gene region of pBR322, then:

    In 1963, two enzymes responsible for restricting the growth of bacteriophage in E.Coli were isolated. They were ……….. and ……… respectively.

    A plasmid containing complete gene copied from human genome is introduced in a bacterial cell, but the bacteria is unable to make a workingcopy of the protein because

    The first restriction endonuclease isolated was

    The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

    DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

    Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

    The DNA molecule to which the gene of interest is integrated for cloning is called

    The cutting of DNA at specific locations became possible with the discovery of

    The separated DNA fragments by gel electrophoresis can be visualized by staining with

    Identify the A, B, C respectively in the following diagram

    Stirred-tank bioreactors have been designed for

    The Taq polymerase enzyme is obtained from

    A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

    Which of the following restriction enzymes produces blunt ends?

    Which of the following is not a component of downstream processing?

    Which of the following is a restriction endonuclease?

    The DNA fragments separated on an agarose gel can be visualised after staining with

    The process of separation and purification of expressed protein formed in heterologous host before marketing is called

    A gene whose expression helps to identify transformed cell is known as

    Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.

    Choose the correct statement/s I. Herbert Boyer worked on Restriction Enzymes of Escherichia coli that cut the DNA to form blunt ends II. Stanley Cohen studied about the nucleoid of E. coli. III. Stanley Cohen developed the technique of isolation and introduction of plasmids into bacterial cells. IV. Herbert Boyer and Stanley Cohen together formed the first genetically modified organism E.coli which was manufacturing plant for antibiotic resistance protein.

    Study the following diagram and identify A, B, C and D. They are respectively as

    This is used as cloning vector in plant genetic engineering

    DNA precipitation out of a mixture of biomolecules can be achieved by treatment with

    Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

    In the following diagram A and B indicate

    The correct sequence of PCR

    Elution is

    This is subjected to insertional inactivation

    A mixture containing DNA fragments A, B, C, and D with molecular weights A + B = C, A > B and C < D was subjected to agarose gel electrophoresis. The position of these fragments from cathode to anode should be

    Study the following gene cloning vector and identify the correct statements. I. Cloning sites are absent in ori region II. tet R has restriction site for BamH I and Sal I III. It is reconstructed cloning vector IV. It has two marker genes.

    The micro particles used in gene gun are made of

    The DNA sequence recognized by Hind II has the length of

    Choose the correct pair from the following :

    In gel electrophoresis, separated DNA fragments can be visualized with the help of :

    Identify the wrong statement with regard to Restriction Enzymes.

    The sequence that controls the copy number of the linked DNA in the vector, is termed :

    The host bacterial cells can be made competent by treating with

    Bioreactors are involved in

    The recombinants lose the resistance to ampicillin if genes are inserted at the following sites of pBR322.

    Match the following techniques or instruments with their usage: (a) Bioreactor (i) Separation of DNA fragments (b) Electrophoresis (ii) Production of large quantities of products (c) PCR (iii) Detection of pathogen, based on antigen-antibody reaction (d) ELISA (iv) Amplification of nucleic acids Select the correct option from following:

    In a mixture, DNA fragments are separated by

    In Recombinant DNA technology antibiotics are used

    First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognizing a specific sequence of six base pairs is

    Select the correct statement from the following :

    Spooling is

    Match the following enzymes with their functions : Column I Column II (a) Restriction endonuclease (i) Joins the DNA fragments (b) Restriction exonuclease (ii) Extends primers on genomic DNA template (c) DNA ligase (iii) Cuts DNA at a specific position (d) Taq polymerase (iv) Removes nucleotides from the ends of DNA Select the correct option from the following :

    The two antibiotic resistance genes on vector pBR322 are for

    A selectable marker is used to:

    An enzyme catalysing the removel of nucleotides from ends of DNA is:

    Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal. (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below

    Restriction endonucleases are :

    In rDNA technology, probes are used for

    Competent bacteria are given thermal shock for forcing the entry of rDNA into host bacteria at the following temperature.

    In a palindromic sequence of bases on a DNA strand is 5ʹ ‘C2T3T1’ 3ʹ. Then 1, 2 and 3 are respectively

    In the construction of first recombinant DNA, the gene encoding for A was introduced into a plasmid of organism B. A and B are respectively.

    Overhangs of DNA fragments formed by the activity of molecular scissor Eco RI, consists of the following base sequence from 3ʹ to 5ʹ direction.

    Match the organism with its use in biotechnology. Column I Column II (a) Restriction endonuclease (i) Helps in visualizing DNA under UV rays (b) Exonucleases (ii) Precipitates out DNA (c) Ethidium bromide (iii) Molecular scissors (d) Chilled ethanol (iv) Remove nucleotides from the ends of the DNA

    Choose the correct pair from the following:

    Identify the wrong statement with regard to Cloning vectors:

    The specific palindromic sequence which is recognized by BamHI is:

    Match Column I with Column II Column I Column II (a) Polymerase Chain Reaction (i) Ampicillin resistant gene (b) Selectable marker (ii) Amplification of DNA (c) Insecticidal protein (iii) Production of large volume of cultures (d) Bioreactors (iv) Cry proteins

    The incorrect statement w.r.t biolistic gun is:

    Nematode-specific genes were introduced into the host plant using:

    If any protein encoding gene is expressed in a heterologous host, it is called a:

    Lysozyme is used during the initial step of recombinant DNA technology:

    Processes of recombinant DNA technology does not essentially involve

    The technique of extracting separated bands of DNA from agarose gel is called:

    Identify the ideal vector for cloning genes in plants.

    In Polymerase Chain Reaction (PCR), primers are used during

    pBR 322 is a cloning vector. It has all except:

    Biosynthetic phase of rDNA technology includes

    Which of the following is correct in context to restriction endonuclease?

    A competent cell is defined as cells:

    The enzyme/s required to obtained naked protoplasts of E. coli during isolation of DNA is

    Study the following figure and identify (a) and (b).

    “The integration of natural science and organism cells, parts thereof, and molecular analogues for products and services”. This definition of biotechnology was given by

    If a protein encoding gene is expressed in a heterologous host, the protein formed is called as

    Which of the following can be categorized as most important feature enabling plasmid to be used as a vector?

    Which of the following enzymes are used to join fragments of DNA?

    The length of palindromic sequence recognized by Eco RI is

    If a gene is inserted in the pBR 322 plasmid by using the enzyme Bam H I, and the rDNA is incorporated into E.coli host. Identify the functions of the marker gene.

    The role of DNA ligase in the consruction of recombinant DNA molecule is

    Which of the following is correctly matched?

    Which of the following is an incorrect statement?

    Which of the following is not a source of restriction endonuclease?

    Which of the following statement is incorrect about gel electrophoresis?

    Which of the following restriction enzymes produce sticky ends?

    The antibiotic resistance gene isolated by Cohen and Boyer was inserted in to native plasmid of which organism?

    The bacteria that possess restriction endonuclease are:

    The source of Taq polymerase used in PCR technique is a

    What is the source of T i plasmid which is modified and used as a cloning vector to deliver the desired genes into plant cells?

    In recombinant DNA technology for the insertion of a foreign gene into a plasmid vector, the vector must be opened by treating it with

    For the large scale production of recombinant protein, we have to use

    In Gel electrophoresis, the substance used as matrix is a

    Identify the restriction sites of pBR322, where if genes are inserted results in the phenomenon insertional inactivation. I. Pvu I II. Pvu II III. Sal I IV. Pst I

    All the features are present in pBR322 except:

    Identify the incorrect statement in context to gel electrophoresis.

    Biolistics or gene gun is a method of transforming host cell with recombinant DNA. In this method, which metallic particles are coated with recombinant DNA?

    Herbert Boyer worked on restriction enzymes of

    Choose the incorrect pair

    The host bacterial cells are made competent to take up rDNA by treating with

    Which of the following are methods of gene transfer in the process of recombinant DNA technology? a. Calcium chloride treatment (bacterial cells) followed by thermal shock. b. Microinjection c. Elution d. Biolistic method

    What you call the technique of separating the desired DNA bands from the electrophoresis run agarose gel?

    What type of enzyme is used in recombinant DNA technology to split a specific sugar phosphate bond in each strand of a DNA double helix ?

    Select the correct statement.

    Which one of the following techniques made it possible to genetically engineer living organism ?

    Which of the following scientists won the ‘Nobel Prize for the development of polymerase chain reaction (PCR) in the year 1993?

    Ligase helps in

    Construction of a recombinant DNA involves

    Which of the following is a method of gene transfer ? a. Micro-injection b. Particle gun c. Electroporation

    Plasmids are used in genetic engineering becasue they are

    The palindromes are groups of letters that form the when read in both direction forward and backward.

    Separation and isolation of DNA fragments can be done by a technique called gel electrophoresis. This technique was developed by

    The specific DNA sequence where Eco- Rl cuts is

    ln plasmid pBR 322, BR stands for

    Restriction endonucleases (Molecular Scissors) are utilised in genetic engineering technique as

    First restriction enzyme was isolated from

    Foreign DNA is also called

    Who discovered recombinant DNA (rDNA) technology ?

    Plasmids are suitable vectors for gene cloning because

    Gel electrophorosis is used for

    T i plasmid used in genetic engineering is obtained from

    Which one of the following techniques made it possible to genetically engineered living organisms ?

    Agarose extracted from sea weeds is used in

    The restriction enzyme(s) used in recombinant DNA technology that make staggered cuts in DNA leaving sticky ends is/are a. Eco RI b. Hind II c. Bam HI

    Which one is a true statement regarding DNA polymerase used in PCR ?

    The usual source of restriction endonucleases used in gene cloning is

    In a genetic engineering experiment, restriction enzymes can be used for

    Heat stable DNA polymerase can be obtained from

    The introduction of tDNA into plants involves

    The taq polymerase enzyme is obtained from

    Which of the following is a restriction endonuclease?

    Stirred-tank bioreactors have been designed for

    Which of the following is not a component of downstream processing?

    What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

    Restriction endonucleases are most commonly obtained from:

    One of the following properties is not the property of a good gene cloning vector used in recombinant DNA technology:

    A plasmid and a linear DNA fragment has 4 restriction sites each for Hind III. Upon treating them with Hind III, how many fragments of plasmid and linear DNA can be formed?

    EFB stands for

    Which amongst the following enzyme is required to remove nucleotides from the ends of the DNA ?

    Technique involving altering the chemistry of genetic material and introducing this into host organisms and thus changing the phenotype of the host organism is referred as

    Which amongst the following statements are correct about gel electrophoresis? (a) Fragments of DNA can be separated by this technique (b) The DNA fragments are separated according to their size through sieving effect (c) The most commonly used matrix is agarose

    The principle of meiosis applied in genetic engineering for the formation of recombinant DNA is

    Which dye is used to stain DNA and visualize it upon exposure to UV radiation?

    Eco RI cuts the DNA between the bases

    Bioreactors are useful in

    Identify the enzyme that is used in polymerase chain reaction.

    Identify the organism that is most extensively used in genetic engineering in the plants.

    Statement A: ‘ rop’ of gene cloning vector pBR322 codes for the proteins involved in the replication of the plasmid. Statement B: In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes.

    Which of the following regions of E. coli cloning vector pBR322, has information for the formation of proteins? A. amp R B. tet R C. Eco R I D. rop

    In the name of a restriction enzyme, which of the following need not always be mentioned?

    The use of PCR in the method of genetic engineering or rDNA technology is

    Read the following statements and find the correct statement.

    The technique used for amplification of DNA is :

    Match Column I with Column II : Column–A Column–B a. Elution i. Cloning vector b. Ampicillin resistant gene ii. Obtaining DNA fragments with sticky ends c. Ti plasmid iii. Selectable marker d. Restriction enzyme iv. Obtaining bands of DNA from agarose gel.

    The integration of natural science and organisms, cells, parts thereof, and molecular analogues for products and service is defined as

    In continuous culture

    Insertion of a gene at which of the following restriction sites of pBR322 does not result in insertional inactivation?

    Biolistics or gene gun method is used in genetic engineering for

    In genetic engineering, restriction enzymes are used for

    A technique used to obtain several copies of a specific segment of DNA accurately and quickly is

    The last step in the method of rDNA technology is

    Select the odd one w.r.t. downstream processing.

    A gene is expressed in a host organism, which does not naturally have this gene or gene fragment. Such a host organism is called as

    Endonucleases was used in genetics engineering as

    The vector that has T-DNA ,

    Restriction enzymes belongs to a large class of the enzymes called

    The speed of migration of ions in an electric field during gel electrophoresis depends on

    The nuclease enzyme, which begins its attack from the free end of a polynucleotide, is called

    Which of the following is not a characteristic feature of the vector pBR322?

    Agarose extracted from sea weeds finds use in :

    The Ti plasmid, is often used for making transgenic plants. The plasmid is found in

    What of the following is not characteristic of the pBR322 vector?

    Select the incorrect match.

    During agarose gel electrophoresis, DNA moves

    During gel electrophoresis, which chemical is used for visualizing the fragmented DNA?

    Restriction endonuclease ( EcoRI ) enzyme cut the strand of DNA

    A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

    What is correct about stirred-tank bioreactors?

    Identify the incorrect statement.

    What is the full form of EFB?

    Select the correct option regarding restriction endonuclease. P. 900 restriction enzymes have been isolated from over 200 strains. Q. The first restriction exonuclease isolated was Hind II. R. Each restriction endonuclease recognizes same palindromic sequence.

    The plsmid pUC derives its name from

    While isolating DNA from bacteria, which of the following enzyme is not used?

    The biological scissors are : i. Restriction enzymes that are used to cut DNA into nucleotide monomers. ii. Enzymes always cut the DNA at specific nucleotide sequences. iii. Present variously recognizing and cutting different DNA sequences. Select the option with the correct statement/s.

    What is true for plasmids?

    Which cell/organism is incorrectly linked with the cell wall lysing enzyme used to extract DNA?

    Select the correct sequence of steps involved in PCR during gene amplification.

    The most commonly used bioreactor is

    GAATTC is a recognition site for the restriction endonuclease

    The most commonly used bioreactor is of stirring type. The stirrer facilitates:

    In the given segment DNA strand the base sequence on the opposite strands are same when read in opposite direction. Such sequences are known as 5’ GAATTCC 3’. 3’ CTTAAG 5’

    Selection incorrect statement.

    The thermostable enzyme, Taq polymerase used in PCR is obtained from

    Restriction endonulease

    Resistance genes for the following are present in pBR322

    The most important feature in a plasmid to be used as a vector is

    The palindromic sequence 5′ GAATTC 3′ is present at which of the following restriction site of the gene cloning vector pBR322 ?

    The stain used to locate the trapped DNA in gel electrophoresis run plate is

    Arrange the three basic steps in genetically modifying an organism in correct sequence.

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