BiologyWhen a PBR322 plasmid is treated with Bam HI to clone a foreign insert successfully, then the colony would be resistant to _A_ and sensitive to _B_. What are A and B ?

When a PBR322 plasmid is treated with Bam HI to clone a foreign insert successfully, then the colony would be resistant to _A_ and sensitive to _B_. What are A and B ?

  1. A

    Ampicillin and tetracyclin

  2. B

    Tetracyclin and ampicillin

  3. C

    Ampicillin and kanamycin

  4. D

    Kanamycin and ampicillin

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    Solution:

    Bam HI recognition sequence is present at tetacyclin resistant gene hence resistance to this antibiotic is lost when a desired gene get attached at this place

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