BiologyWhich of the following is not required for DNA fingerprinting?

Which of the following is not required for DNA fingerprinting?

  1. A
    Isolation of DNA
  2. B
    Digestion of DNA
  3. C
    Hybridisation of DNA
  4. D
    Sequencing of DNA 

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    Solution:

    DNA fingerprinting does not require sequencing of DNA. It involves identification of differences in some specific regions (e.g., VNTR) in DNA. 
    Isolation of DNA from a sample such as blood, saliva, hair, skin cell of the person is the first step in DNA fingerprinting. Digestion of double-stranded DNA is done by restriction endonucleases. Hybridisation of single-stranded DNA with VNTR probe (DNA-DNA hybridisation) helps detect hybrid DNA fragments by autoradiography.
     

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