{"id":145305,"date":"2022-03-20T01:42:07","date_gmt":"2022-03-19T20:12:07","guid":{"rendered":"https:\/\/infinitylearn.com\/surge\/enzyme-linked-immunosorbent-assay-elisa-types-and-principle\/"},"modified":"2025-02-28T15:53:32","modified_gmt":"2025-02-28T10:23:32","slug":"definition-of-elisa-biology-enzyme-linked-immunosorbent-assay","status":"publish","type":"post","link":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/","title":{"rendered":"Definition of ELISA, Enzyme-Linked Immunosorbent Assay (ELISA)"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_37 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><span style=\"display: flex;align-items: center;width: 35px;height: 30px;justify-content: center;\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' style='display:block'><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#Materials_for_ELISA\" title=\"Materials for ELISA\">Materials for ELISA<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#Antigen-antibody_Binding\" title=\"Antigen-antibody Binding\">Antigen-antibody Binding<\/a><ul class='ez-toc-list-level-3'><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#Principle_of_Conservation_of_Momentum\" title=\"Principle of Conservation of Momentum\">Principle of Conservation of Momentum<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#Types_of_ELISA_Assay\" title=\"Types of ELISA Assay\">Types of ELISA Assay<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<p>ELISA stands for enzyme-linked immunosorbent assay. It is a biochemical technique used to detect a specific antigen in a sample. The antigen is first immobilized on a solid surface. A serum sample is then added to the antigen. If the antigen is present in the serum, it will bind to the immobilized antigen. An enzyme-linked antibody is then added to the mixture. If the antigen is present in the serum, it will bind to the enzyme-linked antibody. The presence of the enzyme-linked antibody can be detected by various means, such as a change in color or light production. <span style=\"font-size: 12pt;\">Definition of ELISA.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Materials_for_ELISA\"><\/span>Materials for ELISA<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The materials for ELISA include the ELISA plate, the sample, the antibody, the enzyme-linked marker, and the substrate. The ELISA plate is a flat, round dish coated with the antibody. The sample is added to the plate, and the antibody binds to the target molecule in the sample. The enzyme-linked marker is then added, and it binds to the antibody. The substrate is then added, and it binds to the enzyme-linked marker. When the substrate is added, it is converted to a colored product that can be easily measured.<\/p>\n<p>&nbsp;<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Antigen-antibody_Binding\"><\/span>Antigen-antibody Binding<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>When an <a href=\"https:\/\/infinitylearn.com\/surge\/biology\/antigen\/\">antigen<\/a> is introduced into the body, it will bind to antibodies present on the surface of B-cells. This interaction between antigen and antibody is very specific, and the antigen will only bind to a particular antibody specifically designed to interact with it. This interaction between antigen and antibody is known as an antigen-antibody binding pair.<\/p>\n<h3>Principle of Conservation of Momentum<\/h3>\n<p>The principle of conservation of momentum states that the total momentum of a system is constant. This means that the total momentum before an event equals the total momentum after the event. Momentum is a measure of the motion of an object. It is the product of mass and velocity.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"Types_of_ELISA_Assay\"><\/span>Types of ELISA Assay<span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p>ELISA assay is a biochemical technique used to detect and quantify the presence of a target molecule in a sample. The assay involves the immobilization of the target molecule onto a solid surface, such as a microtiter plate, and the subsequent addition of a labeled detection molecule. After incubation, the amount of detection molecule bound to the target molecule is quantified by measuring the signal generated by the labeled molecule. A spectrophotometric or fluorometric assay typically generates the signal.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ELISA stands for enzyme-linked immunosorbent assay. It is a biochemical technique used to detect a specific antigen in a sample. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_focuskw":"Definition of ELISA","_yoast_wpseo_title":"Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained","_yoast_wpseo_metadesc":"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.","custom_permalink":"biology\/enzyme-linked-immunosorbent-assay\/"},"categories":[11],"tags":[],"table_tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained<\/title>\n<meta name=\"description\" content=\"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained\" \/>\n<meta property=\"og:description\" content=\"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/\" \/>\n<meta property=\"og:site_name\" content=\"Infinity Learn by Sri Chaitanya\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/\" \/>\n<meta property=\"article:published_time\" content=\"2022-03-19T20:12:07+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-02-28T10:23:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2025\/04\/infinitylearn.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"1008\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@InfinityLearn_\" \/>\n<meta name=\"twitter:site\" content=\"@InfinityLearn_\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"vipin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained","description":"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/","og_locale":"en_US","og_type":"article","og_title":"Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained","og_description":"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.","og_url":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/","og_site_name":"Infinity Learn by Sri Chaitanya","article_publisher":"https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/","article_published_time":"2022-03-19T20:12:07+00:00","article_modified_time":"2025-02-28T10:23:32+00:00","og_image":[{"width":1920,"height":1008,"url":"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2025\/04\/infinitylearn.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_creator":"@InfinityLearn_","twitter_site":"@InfinityLearn_","twitter_misc":{"Written by":"vipin","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Organization","@id":"https:\/\/infinitylearn.com\/surge\/#organization","name":"Infinity Learn","url":"https:\/\/infinitylearn.com\/surge\/","sameAs":["https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/","https:\/\/www.instagram.com\/infinitylearn_by_srichaitanya\/","https:\/\/www.linkedin.com\/company\/infinity-learn-by-sri-chaitanya\/","https:\/\/www.youtube.com\/c\/InfinityLearnEdu","https:\/\/twitter.com\/InfinityLearn_"],"logo":{"@type":"ImageObject","@id":"https:\/\/infinitylearn.com\/surge\/#logo","inLanguage":"en-US","url":"","contentUrl":"","caption":"Infinity Learn"},"image":{"@id":"https:\/\/infinitylearn.com\/surge\/#logo"}},{"@type":"WebSite","@id":"https:\/\/infinitylearn.com\/surge\/#website","url":"https:\/\/infinitylearn.com\/surge\/","name":"Infinity Learn by Sri Chaitanya","description":"Surge","publisher":{"@id":"https:\/\/infinitylearn.com\/surge\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/infinitylearn.com\/surge\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#webpage","url":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/","name":"Understanding ELISA: Enzyme-Linked Immunosorbent Assay Explained","isPartOf":{"@id":"https:\/\/infinitylearn.com\/surge\/#website"},"datePublished":"2022-03-19T20:12:07+00:00","dateModified":"2025-02-28T10:23:32+00:00","description":"Learn the Definition and Key Concepts of ELISA (Enzyme-Linked Immunosorbent Assay). Discover its uses, procedure, and significance in diagnostics and research.","breadcrumb":{"@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/infinitylearn.com\/surge\/"},{"@type":"ListItem","position":2,"name":"Definition of ELISA, Enzyme-Linked Immunosorbent Assay (ELISA)"}]},{"@type":"Article","@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#article","isPartOf":{"@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#webpage"},"author":{"@id":"https:\/\/infinitylearn.com\/surge\/#\/schema\/person\/d931698bc4645b2739855720864f30e2"},"headline":"Definition of ELISA, Enzyme-Linked Immunosorbent Assay (ELISA)","datePublished":"2022-03-19T20:12:07+00:00","dateModified":"2025-02-28T10:23:32+00:00","mainEntityOfPage":{"@id":"https:\/\/infinitylearn.com\/surge\/biology\/enzyme-linked-immunosorbent-assay\/#webpage"},"wordCount":398,"publisher":{"@id":"https:\/\/infinitylearn.com\/surge\/#organization"},"articleSection":["Biology"],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/infinitylearn.com\/surge\/#\/schema\/person\/d931698bc4645b2739855720864f30e2","name":"vipin","image":{"@type":"ImageObject","@id":"https:\/\/infinitylearn.com\/surge\/#personlogo","inLanguage":"en-US","url":"https:\/\/secure.gravatar.com\/avatar\/c9a84adf9d11e7ad01332089c3e52538?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c9a84adf9d11e7ad01332089c3e52538?s=96&d=mm&r=g","caption":"vipin"},"sameAs":["http:\/\/surge.infinitylearn.com"],"url":"https:\/\/infinitylearn.com\/surge\/author\/vipin\/"}]}},"_links":{"self":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts\/145305"}],"collection":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/comments?post=145305"}],"version-history":[{"count":0,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts\/145305\/revisions"}],"wp:attachment":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/media?parent=145305"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/categories?post=145305"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/tags?post=145305"},{"taxonomy":"table_tags","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/table_tags?post=145305"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}