{"id":147115,"date":"2022-03-20T04:12:57","date_gmt":"2022-03-19T22:42:57","guid":{"rendered":"https:\/\/infinitylearn.com\/surge\/insertional-inactivation-introduction-use-and-method\/"},"modified":"2022-10-23T18:36:26","modified_gmt":"2022-10-23T13:06:26","slug":"insertional-inactivation-introduction-use-and-method","status":"publish","type":"post","link":"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/","title":{"rendered":"Insertional Inactivation &#8211; Introduction, Use and Method"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_37 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><span style=\"display: flex;align-items: center;width: 35px;height: 30px;justify-content: center;\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' style='display:block'><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/#Introduction_to_Insertional_Inactivation\" title=\"Introduction to Insertional Inactivation\">Introduction to Insertional Inactivation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/#What_is_Meant_by_Insertional_Inactivation\" title=\"What is Meant by Insertional Inactivation?\">What is Meant by Insertional Inactivation?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/#Requirements_in_r-DNA_Technology\" title=\"Requirements in r-DNA Technology\">Requirements in r-DNA Technology<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/#The_Use_of_an_Insertional_Inactivation_Process\" title=\"The Use of an Insertional Inactivation Process\">The Use of an Insertional Inactivation Process<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/infinitylearn.com\/surge\/biology\/insertional-inactivation\/#Insertional_Inactivation_Method\" title=\"Insertional Inactivation Method\">Insertional Inactivation Method<\/a><\/li><\/ul><\/nav><\/div>\n<h2><\/h2>\n<h2><span class=\"ez-toc-section\" id=\"Introduction_to_Insertional_Inactivation\"><\/span>Introduction to Insertional Inactivation<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Insertional inactivation is a technique used to inactivate genes by inserting a DNA fragment into the gene&#8217;s promoter region. This technique can be used to study the function of a gene or to disrupt its function. Insertional Inactivation &#8211; Introduction Use and Method.<\/p>\n<p><img loading=\"lazy\" class=\"aligncenter wp-image-147114 size-full\" src=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/insertional-inactivation-introduction-use-and-method.jpg\" alt=\"Insertional Inactivation - Introduction Use and Method\" width=\"606\" height=\"428\" srcset=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/insertional-inactivation-introduction-use-and-method.jpg?v=1647729774 606w, https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/insertional-inactivation-introduction-use-and-method-300x212.jpg?v=1647729774 300w\" sizes=\"(max-width: 606px) 100vw, 606px\" \/><\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Meant_by_Insertional_Inactivation\"><\/span>What is Meant by Insertional Inactivation?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Insertional inactivation is a process by which a gene is inactivated by the insertion of a new piece of DNA into it. This can occur naturally, as in the case of transposons, or can be done artificially, as in the case of gene therapy.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Requirements_in_r-DNA_Technology\"><\/span>Requirements in r-DNA Technology<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>In recombinant DNA technology, also called genetic engineering, DNA is moved from one organism to another. This process is used to create genetically modified organisms (GMOs) or to create new DNA sequences. The technology is used to produce drugs, food, and other products.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"The_Use_of_an_Insertional_Inactivation_Process\"><\/span>The Use of an Insertional Inactivation Process<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Inactivation processes can be used to inactivate a gene of interest. One such process is insertional inactivation. In this process, a gene of interest is inactivated by inserting a DNA sequence that disrupts its function into its gene sequence. This can be done using a vector such as a plasmid or viral vector. The vector is introduced into a cell, and the gene of interest is disrupted by the insertion of the DNA sequence. This process can be used to inactivate a gene permanently or transiently.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Insertional_Inactivation_Method\"><\/span>Insertional Inactivation Method<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The insertional inactivation method is a technique used to inactivate a gene by disrupting its function by inserting a piece of DNA into it.<\/p>\n<p>The inserted DNA can be a gene of interest that is to be knocked out, or it can be a random piece of DNA that is used to scramble the gene&#8217;s function. This method can be used to study the function of a gene, or to create mutant strains of organisms.<\/p>\n<p>Insertional Inactivation &#8211; Introduction Use and Method.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Introduction to Insertional Inactivation Insertional inactivation is a technique used to inactivate genes by inserting a DNA fragment into the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_focuskw":"Insertional Inactivation - Introduction Use and Method","_yoast_wpseo_title":"","_yoast_wpseo_metadesc":"Learn about Insertional Inactivation - Introduction Use and Method topic in details explained by subject experts on infinitylearn.com.","custom_permalink":"biology\/insertional-inactivation\/"},"categories":[11],"tags":[],"table_tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Insertional Inactivation - 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