{"id":148572,"date":"2022-03-21T09:24:22","date_gmt":"2022-03-21T03:54:22","guid":{"rendered":"https:\/\/infinitylearn.com\/surge\/elution-chromatography-graph-types-time-and-volume\/"},"modified":"2024-12-13T16:16:06","modified_gmt":"2024-12-13T10:46:06","slug":"elution-chromatography-graph-types-time-and-volume","status":"publish","type":"post","link":"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/","title":{"rendered":"Elution Chromatography &#8211; Graph, Types, Time and Volume"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_37 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><span style=\"display: flex;align-items: center;width: 35px;height: 30px;justify-content: center;\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' style='display:block'><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#What_is_Elution_Chromatography\" title=\"What is Elution Chromatography?\">What is Elution Chromatography?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#Elution_Chromatography_Graph\" title=\"Elution Chromatography Graph\">Elution Chromatography Graph<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#What_is_Elution\" title=\"What is Elution?\">What is Elution?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#Elution_Time_and_Volume\" title=\"Elution Time and Volume\">Elution Time and Volume<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#Types_of_Elution_Chromatography\" title=\"Types of Elution Chromatography\">Types of Elution Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#Efficiency_of_Column_Elution_Chromatography\" title=\"Efficiency of Column Elution Chromatography\">Efficiency of Column Elution Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#5_What_Is_Elution\" title=\"5. What Is Elution?\">5. What Is Elution?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#6_How_Can_Elution_Chromatography_Be_Described\" title=\"6. How Can Elution Chromatography Be Described?\">6. How Can Elution Chromatography Be Described?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#7_Time_of_Retention\" title=\"7. Time of Retention?\">7. Time of Retention?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/#8_Constant_of_Equilibrium\" title=\"8. Constant of Equilibrium?\">8. Constant of Equilibrium?<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Elution_Chromatography\"><\/span>What is Elution Chromatography?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Elution chromatography is a type of chromatography that uses a mobile phase (liquid or gas) to move the sample mixture through a stationary phase (solid or liquid). The mobile phase can be used to elute (remove) the sample from the stationary phase. Elution chromatography is used to separate components of a mixture by their relative affinities for the two phases. Elution Chromatography &#8211; Graph Types Time and Volume.<\/p>\n<p><img loading=\"lazy\" class=\"aligncenter wp-image-148571 size-full\" src=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/elution-chromatography-graph-types-time-and-volume.jpg\" alt=\"Elution Chromatography - Graph, Types, Time and Volume\" width=\"606\" height=\"428\" srcset=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/elution-chromatography-graph-types-time-and-volume.jpg?v=1647834859 606w, https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/elution-chromatography-graph-types-time-and-volume-300x212.jpg?v=1647834859 300w\" sizes=\"(max-width: 606px) 100vw, 606px\" \/><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Elution_Chromatography_Graph\"><\/span>Elution Chromatography Graph<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The elution chromatography graph shows the different compounds that were present in a sample. Each compound is represented by a different color, and the intensity of the color corresponds to the amount of that compound in the sample.<\/p>\n<p>The graph shows that the sample contains a number of different compounds, including several different colors. The blue compound is the most prevalent, followed by the green and red compounds. There is also a small amount of the yellow compound present in the sample.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Elution\"><\/span>What is Elution?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Elution is the process of removing a substance from a solid matrix. For example, if you have a sample of soil that contains a contaminant, you can use elution to remove the contaminant from the soil.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Elution_Time_and_Volume\"><\/span>Elution Time and Volume<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>This assay uses a 96-well plate. Each well will require 100 \u03bcL of sample.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_Elution_Chromatography\"><\/span>Types of Elution Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>There are many types of elution <a href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/gas-chromatography\/\">chromatography<\/a>. The two most common types are ion exchange chromatography and reverse phase chromatography.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Efficiency_of_Column_Elution_Chromatography\"><\/span>Efficiency of Column Elution Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The efficiency of column elution chromatography is determined by the number of theoretical plates that can be achieved. The number of theoretical plates is determined by the following equation:<\/p>\n<p>N = (2 L\/\u0394P) (C\/\u0394P)<\/p>\n<p>Where:<\/p>\n<p>N = number of theoretical plates<\/p>\n<p>L = length of column<\/p>\n<p>\u0394P = pressure drop<\/p>\n<p>C = concentration of solute<\/p>\n<h2><span class=\"ez-toc-section\" id=\"5_What_Is_Elution\"><\/span>5. What Is Elution?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Elution is the process of removing a substance from a solid support.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"6_How_Can_Elution_Chromatography_Be_Described\"><\/span>6. How Can Elution Chromatography Be Described?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Elution chromatography is a type of chromatography that uses a solvent to move the desired compound out of a sample. The solvent is called the eluent.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"7_Time_of_Retention\"><\/span>7. Time of Retention?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>One year.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"8_Constant_of_Equilibrium\"><\/span>8. Constant of Equilibrium?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The constant of equilibrium is the equilibrium constant.<\/p>\n<p>Elution Chromatography &#8211; Graph Types Time and Volume.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is Elution Chromatography? Elution chromatography is a type of chromatography that uses a mobile phase (liquid or gas) to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_focuskw":"Elution Chromatography - Graph Types Time and Volume","_yoast_wpseo_title":"","_yoast_wpseo_metadesc":"Learn about Elution Chromatography - Graph Types Time and Volume explained by subject experts on infinitylearn.com.","custom_permalink":"chemistry\/elution-chromatography\/"},"categories":[1],"tags":[],"table_tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Elution Chromatography - Graph, Types, Time and Volume - Infinity Learn by Sri Chaitanya<\/title>\n<meta name=\"description\" content=\"Learn about Elution Chromatography - Graph Types Time and Volume explained by subject experts on infinitylearn.com.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/elution-chromatography\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Elution Chromatography - 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