{"id":149492,"date":"2022-03-21T10:25:20","date_gmt":"2022-03-21T04:55:20","guid":{"rendered":"https:\/\/infinitylearn.com\/surge\/partition-chromatography-principle-diagram-types-and-applications\/"},"modified":"2024-12-13T15:05:41","modified_gmt":"2024-12-13T09:35:41","slug":"partition-chromatography-principle-diagram-types-and-applications","status":"publish","type":"post","link":"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/","title":{"rendered":"Partition Chromatography &#8211; Principle, Diagram, Types and Applications"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_37 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><span style=\"display: flex;align-items: center;width: 35px;height: 30px;justify-content: center;\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' style='display:block'><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#What_is_Chromatography\" title=\"What is Chromatography?\">What is Chromatography?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#What_is_Partition_Chromatography\" title=\"What is Partition Chromatography?\">What is Partition Chromatography?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Principle_of_Chromatography\" title=\"Principle of Chromatography\">Principle of Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Partition_Chromatography_Principle\" title=\"Partition Chromatography Principle\">Partition Chromatography Principle<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Paper_Chromatography_Diagram_-_A_type_of_Partition_Chromatography\" title=\"Paper Chromatography Diagram &#8211; A type of Partition Chromatography\">Paper Chromatography Diagram &#8211; A type of Partition Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Types_of_Partition_Chromatography\" title=\"Types of Partition Chromatography\">Types of Partition Chromatography<\/a><ul class='ez-toc-list-level-4'><li class='ez-toc-heading-level-4'><ul class='ez-toc-list-level-4'><li class='ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Ion-exchange_chromatography\" title=\"Ion-exchange chromatography\">Ion-exchange chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Affinity_chromatography\" title=\"Affinity chromatography\">Affinity chromatography<\/a><\/li><\/ul><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Applications_of_Partition_Chromatography\" title=\"Applications of Partition Chromatography\">Applications of Partition Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/#Partition_Chromatography_and_How_to_study_it\" title=\"Partition Chromatography and How to study it?\">Partition Chromatography and How to study it?<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Chromatography\"><\/span>What is Chromatography?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Partition Chromatography: <a href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/partition-chromatography\/\">Chromatography<\/a> is a technique used to separate the components of a mixture. The mixture is placed on a stationary phase and a mobile phase is used to move the components of the mixture through the stationary phase. The different components of the mixture will move through the stationary phase at different rates, allowing them to be separated.<\/p>\n<p><img loading=\"lazy\" class=\"aligncenter wp-image-149491 size-full\" src=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/partition-chromatography-principle-diagram-types-and-applications.jpg\" alt=\"Partition Chromatography - Principle, Diagram, Types and Applications\" width=\"606\" height=\"428\" srcset=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/partition-chromatography-principle-diagram-types-and-applications.jpg?v=1647838515 606w, https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/partition-chromatography-principle-diagram-types-and-applications-300x212.jpg?v=1647838515 300w\" sizes=\"(max-width: 606px) 100vw, 606px\" \/><\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Partition_Chromatography\"><\/span>What is Partition Chromatography?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Partition chromatography is a separation technique in which a mixture dissolved in a solvent and then passed through a filter. The separated components of the mixture then recovered from the filter.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Principle_of_Chromatography\"><\/span>Principle of Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A principle of chromatography is that different molecules in a mixture will travel through a stationary phase at different rates. This is because different molecules have different sizes, shapes, and polarities. The different molecules will also interact differently with the stationary phase.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Partition_Chromatography_Principle\"><\/span>Partition Chromatography Principle<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The partition chromatography principle states that a solute will distribute itself between two immiscible liquids according to its distribution coefficient. The higher the distribution coefficient, the more the solute will partition into the more concentrated liquid.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Paper_Chromatography_Diagram_-_A_type_of_Partition_Chromatography\"><\/span>Paper Chromatography Diagram &#8211; A type of Partition Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A type of Partition Chromatography in which the stationary phase is a sheet of paper. The mobile phase is a liquid, which drawn up the paper by capillary action. Therefore the different components of the mixture drawn up the paper at different rates, according to their relative solubilities in the two phases. This produces a series of colored bands, which can used to identify the different components of the mixture.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_Partition_Chromatography\"><\/span>Types of Partition Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>There are three types of partition chromatography:<\/p>\n<h4><span class=\"ez-toc-section\" id=\"Ion-exchange_chromatography\"><\/span>Ion-exchange chromatography<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>Ion-exchange chromatography is a type of partition chromatography that uses a stationary phase that made up of ion-exchange resins. These resins made up of small, positively charged beads. The molecules that separated placed in a liquid solution that also mixed with a salt. The salt will bind to the small, positively charged beads on the resin. The molecules that separated will then pulled through the resin by the salt.<\/p>\n<p>The molecules that pulled through the resin will separated based on how well they bind to the resin. Molecules that bind best to the resin will the most tightly attached to the resin and will the last to pulled through. The molecules that bind the least to the resin will the first to pulled through.<\/p>\n<p>This type of chromatography used to separate molecules based on their charge. Therefore the most common application of this type of chromatography is the separation of proteins based on their charge.<\/p>\n<h4><span class=\"ez-toc-section\" id=\"Affinity_chromatography\"><\/span>Affinity chromatography<span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p>Affinity chromatography is a type of partition chromatography that uses a stationary phase that made up of antibodies or other proteins that have a specific affinity for a certain molecule. The molecules that separated placed in a liquid solution that also mixed with the antibody or protein. The antibody or protein will bind to the molecule<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Applications_of_Partition_Chromatography\"><\/span>Applications of Partition Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Some possible applications of partition chromatography include the separation of proteins, carbohydrates, and lipids. Partition chromatography can also used to purify drugs and other molecules.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Partition_Chromatography_and_How_to_study_it\"><\/span>Partition Chromatography and How to study it?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A method of separation in which a mixture dissolved in a solvent and the components then separated by passing the mixture through a filter. The components then collected based on their different solubilities in the two phases.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is Chromatography? Partition Chromatography: Chromatography is a technique used to separate the components of a mixture. The mixture is [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_focuskw":"Partition Chromatography","_yoast_wpseo_title":"%%title%% %%page%%","_yoast_wpseo_metadesc":"Learn Partition Chromatography topic of Chemistry in details explained by subject experts on infinitylearn.com. 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