{"id":150871,"date":"2022-03-21T11:54:54","date_gmt":"2022-03-21T06:24:54","guid":{"rendered":"https:\/\/infinitylearn.com\/surge\/centrifugation-significance-general-applications-and-principle\/"},"modified":"2025-07-24T11:47:32","modified_gmt":"2025-07-24T06:17:32","slug":"centrifugation-significance-general-applications-and-principle","status":"publish","type":"post","link":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/","title":{"rendered":"Centrifugation \u2013 Significance, General Applications and Principle"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_37 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><span style=\"display: flex;align-items: center;width: 35px;height: 30px;justify-content: center;\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1' style='display:block'><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#What_is_Centrifugation\" title=\"What is Centrifugation?\">What is Centrifugation?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#What_is_Centrifugation-2\" title=\"What is Centrifugation?\">What is Centrifugation?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#Centrifugal_Force\" title=\"Centrifugal Force\">Centrifugal Force<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#General_Applications_of_Centrifugation\" title=\"General Applications of Centrifugation\">General Applications of Centrifugation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#What_is_a_Centrifuge\" title=\"What is a Centrifuge?\">What is a Centrifuge?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#Usage_of_a_Centrifuge\" title=\"Usage of a Centrifuge\">Usage of a Centrifuge<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#Radial_Acceleration\" title=\"Radial Acceleration\">Radial Acceleration<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#Types_of_Centrifugation\" title=\"Types of Centrifugation\">Types of Centrifugation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#What_is_the_Gradient\" title=\"What is the Gradient?\">What is the Gradient?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#Principles_of_Centrifugation\" title=\"Principles of Centrifugation\">Principles of Centrifugation<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Centrifugation\"><\/span>What is Centrifugation?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Centrifugation \u2013 Significance: <a href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/\">Centrifugation<\/a> is a technique employed in biochemistry and molecular biology to separate macromolecules, particles, and precipitates from solutions. The technique exploits the force of gravity acting on a rotating cylinder or disk to sediment objects of greater density to the bottom of the container.<\/p>\n<p><img loading=\"lazy\" class=\"aligncenter wp-image-150870 size-full\" src=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg\" alt=\"Centrifugation \u2013 Significance, General Applications and Principle\" width=\"606\" height=\"428\" srcset=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg?v=1647969060 606w, https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle-300x212.jpg?v=1647969060 300w\" sizes=\"(max-width: 606px) 100vw, 606px\" \/><\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_Centrifugation-2\"><\/span>What is Centrifugation?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Centrifugation is a laboratory technique that employs centrifugal force to separate particles from a suspension. The particles are accelerated by the centrifugal force and move to the outer edge of the centrifuge tube. The speed of the centrifuge and the size of the tube determine the separation. The heavy particles move to the outside of the tube while the light particles remain in the center.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Centrifugal_Force\"><\/span>Centrifugal Force<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Centrifugal force is a fictitious force that is said to act outwardly in a rotating frame of reference. It is the result of the inertia of rotating objects. In the absence of an external force, a rotating object will continue to rotate because of its own inertia. The centrifugal force is the outward force that is said to act on the object.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"General_Applications_of_Centrifugation\"><\/span>General Applications of Centrifugation<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Centrifugation is a technique used to separate particles from a liquid suspension by exploiting their differences in mass and density. When a liquid is placed in a centrifuge and spun at high speed, the particles in the liquid are forced to the outer edge of the container. The heavier and denser particles are forced to the bottom while the lighter and less dense particles are forced to the top. This technique can be used to separate cells, viruses, proteins, and other molecules from a liquid sample.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_a_Centrifuge\"><\/span>What is a Centrifuge?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A centrifuge is a machine that separates substances by spinning them around a fixed axis. The centrifuge causes the heavier substances to move outward while the lighter substances remain in the center.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Usage_of_a_Centrifuge\"><\/span>Usage of a Centrifuge<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>A centrifuge is a device that uses centrifugal force to separate particles from a liquid or to compact them.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Radial_Acceleration\"><\/span>Radial Acceleration<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The radial acceleration is the component of the acceleration vector that points outward from the center of rotation. This acceleration is responsible for the circular motion of objects around a central point.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_Centrifugation\"><\/span>Types of Centrifugation<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>There are different types of centrifugation that are used for different applications.<\/p>\n<ul>\n<li>Ultracentrifugation is a type of centrifugation that uses very high speeds to separate molecules by weight.<\/li>\n<li>Pellet centrifugation is a type of centrifugation that is used to separate particles by size.<\/li>\n<li>Gel centrifugation is a type of centrifugation that is used to separate molecules by size.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"What_is_the_Gradient\"><\/span>What is the Gradient?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The gradient is the change in the value of a function at a given point. The gradient is usually represented using the symbol \u2207.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Principles_of_Centrifugation\"><\/span>Principles of Centrifugation<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Centrifugation is a separation technique that uses centrifugal force to separate particles from a liquid or suspension. The technique is based on the principle that different particles have different masses and will be affected differently by the force of gravity. Lighter particles will be moved to the outside of the container while heavier particles will be moved to the center.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is Centrifugation? Centrifugation \u2013 Significance: Centrifugation is a technique employed in biochemistry and molecular biology to separate macromolecules, particles, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_yoast_wpseo_focuskw":"Centrifugation \u2013 Significance","_yoast_wpseo_title":"Centrifugation \u2013 Principle, Uses & Importance in Science and Industry","_yoast_wpseo_metadesc":"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions","custom_permalink":"chemistry\/centrifugation\/"},"categories":[1],"tags":[],"table_tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Centrifugation \u2013 Principle, Uses &amp; Importance in Science and Industry<\/title>\n<meta name=\"description\" content=\"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Centrifugation \u2013 Principle, Uses &amp; Importance in Science and Industry\" \/>\n<meta property=\"og:description\" content=\"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions\" \/>\n<meta property=\"og:url\" content=\"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/\" \/>\n<meta property=\"og:site_name\" content=\"Infinity Learn by Sri Chaitanya\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/\" \/>\n<meta property=\"article:published_time\" content=\"2022-03-21T06:24:54+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-07-24T06:17:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@InfinityLearn_\" \/>\n<meta name=\"twitter:site\" content=\"@InfinityLearn_\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"vipin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Centrifugation \u2013 Principle, Uses & Importance in Science and Industry","description":"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/","og_locale":"en_US","og_type":"article","og_title":"Centrifugation \u2013 Principle, Uses & Importance in Science and Industry","og_description":"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions","og_url":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/","og_site_name":"Infinity Learn by Sri Chaitanya","article_publisher":"https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/","article_published_time":"2022-03-21T06:24:54+00:00","article_modified_time":"2025-07-24T06:17:32+00:00","og_image":[{"url":"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg"}],"twitter_card":"summary_large_image","twitter_creator":"@InfinityLearn_","twitter_site":"@InfinityLearn_","twitter_misc":{"Written by":"vipin","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Organization","@id":"https:\/\/infinitylearn.com\/surge\/#organization","name":"Infinity Learn","url":"https:\/\/infinitylearn.com\/surge\/","sameAs":["https:\/\/www.facebook.com\/InfinityLearn.SriChaitanya\/","https:\/\/www.instagram.com\/infinitylearn_by_srichaitanya\/","https:\/\/www.linkedin.com\/company\/infinity-learn-by-sri-chaitanya\/","https:\/\/www.youtube.com\/c\/InfinityLearnEdu","https:\/\/twitter.com\/InfinityLearn_"],"logo":{"@type":"ImageObject","@id":"https:\/\/infinitylearn.com\/surge\/#logo","inLanguage":"en-US","url":"","contentUrl":"","caption":"Infinity Learn"},"image":{"@id":"https:\/\/infinitylearn.com\/surge\/#logo"}},{"@type":"WebSite","@id":"https:\/\/infinitylearn.com\/surge\/#website","url":"https:\/\/infinitylearn.com\/surge\/","name":"Infinity Learn by Sri Chaitanya","description":"Surge","publisher":{"@id":"https:\/\/infinitylearn.com\/surge\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/infinitylearn.com\/surge\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"ImageObject","@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#primaryimage","inLanguage":"en-US","url":"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg?v=1647969060","contentUrl":"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg?v=1647969060","width":606,"height":428},{"@type":"WebPage","@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#webpage","url":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/","name":"Centrifugation \u2013 Principle, Uses & Importance in Science and Industry","isPartOf":{"@id":"https:\/\/infinitylearn.com\/surge\/#website"},"primaryImageOfPage":{"@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#primaryimage"},"datePublished":"2022-03-21T06:24:54+00:00","dateModified":"2025-07-24T06:17:32+00:00","description":"Centrifugation is technique employed in biochemistry and molecular biology to separate macromolecules, particles, precipitates from solutions","breadcrumb":{"@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/infinitylearn.com\/surge\/"},{"@type":"ListItem","position":2,"name":"Centrifugation \u2013 Significance, General Applications and Principle"}]},{"@type":"Article","@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#article","isPartOf":{"@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#webpage"},"author":{"@id":"https:\/\/infinitylearn.com\/surge\/#\/schema\/person\/d931698bc4645b2739855720864f30e2"},"headline":"Centrifugation \u2013 Significance, General Applications and Principle","datePublished":"2022-03-21T06:24:54+00:00","dateModified":"2025-07-24T06:17:32+00:00","mainEntityOfPage":{"@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#webpage"},"wordCount":521,"publisher":{"@id":"https:\/\/infinitylearn.com\/surge\/#organization"},"image":{"@id":"https:\/\/infinitylearn.com\/surge\/chemistry\/centrifugation\/#primaryimage"},"thumbnailUrl":"https:\/\/infinitylearn.com\/surge\/wp-content\/uploads\/2022\/03\/centrifugation-significance-general-applications-and-principle.jpg","inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/infinitylearn.com\/surge\/#\/schema\/person\/d931698bc4645b2739855720864f30e2","name":"vipin","image":{"@type":"ImageObject","@id":"https:\/\/infinitylearn.com\/surge\/#personlogo","inLanguage":"en-US","url":"https:\/\/secure.gravatar.com\/avatar\/c9a84adf9d11e7ad01332089c3e52538?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c9a84adf9d11e7ad01332089c3e52538?s=96&d=mm&r=g","caption":"vipin"},"sameAs":["http:\/\/surge.infinitylearn.com"],"url":"https:\/\/infinitylearn.com\/surge\/author\/vipin\/"}]}},"_links":{"self":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts\/150871"}],"collection":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/comments?post=150871"}],"version-history":[{"count":1,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts\/150871\/revisions"}],"predecessor-version":[{"id":771371,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/posts\/150871\/revisions\/771371"}],"wp:attachment":[{"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/media?parent=150871"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/categories?post=150871"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/tags?post=150871"},{"taxonomy":"table_tags","embeddable":true,"href":"https:\/\/infinitylearn.com\/surge\/wp-json\/wp\/v2\/table_tags?post=150871"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}