First slide

Tools of Recombinant DNA Technology

Question

Identify correct statement in context to gel electrophoresis.

Easy

A

Larger DNA fragments will move farther on agarose gel.
B

Smaller DNA fragments will travel less on agarose gel
C

Largest DNA fragments will be seen close to anode.
D

DNA is loaded towards the cathode end.

Solution

Large DNA fragments travel less and smaller travel more on agarose gel.
Largest DNA fragments are found close to cathode and shortest towards the anode.
DNA being negatively charged is loaded towards cathode so that positive charge on anode can force the fragments to travel on agarose gel.

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