Q.

A competent cell is defined as cells

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a

those which are given a heat shock at 420 C for 45 seconds

b

the cells that show susceptibility to tetracyclin

c

the cell that are resistant to ampicillin

d

those which are treated with calcium chloride

answer is B.

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Detailed Solution

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In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. This is done by treating them with a specific concentration of a divalent cation, such as calcium, which increases the efficiency with which DNA enters the bacterium through pores in its cell wall.

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