(a) Explain the significance of ‘palindromic nucleotide sequence’ in the formation of recombinant DNA.
(b) Write the use of restriction endonuclease in the above process.

a) Palindromic sequence is present in nucleic acids. They read the same sequence from 5’ to 3’ direction and from 3’ to 5’ direction. Example, 5' GAATTC 3' / 3' CTTAAG 5'. Palindromic sequences are important because these are the sites of action of endonucleases.

b) Restriction enzymes cleave the double-stranded nucleic acids at specific sites called palindromic sequences.