(a) How are recombinant vectors created?
(b) For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.

(a) Steps for creating recombinant vectors are:

i. Restriction enzyme is used to cut desired DNA segments at a particular restriction site.

ii. DNA ligase is used to linked the plasmid DNA with alien leads to formation of the recombinant vector.

(b) One type of restriction endonuclease is used to create one recombinant vector as it recognises and cut desired DNA at a specific sequence, for creating that only one RE is used. If more than one RE is used it will create many cleavage and will complicate gene cloning.