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A mixture contains three amino acids A (pH = 3.2), B (pH = 5.7) and C (pH = 9.7).
When it is subjected to electrophoresis at pH 7 .7 , in which direction will each component of the mixture move?

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A to anode, B and C to cathode

A to anode, B no movement, C to cathode

A and B to anode and C to cathode

A to cathode, B no movement, C to anode

answer is C.

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Detailed Solution

Since pH of A and B is less than pH (7.7), so they will exist in anionic form and will migrate towards anode.
Since pH of C is greater than the pH of medium, so it will exist in cationic form and migrate towards cathode.

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