Q.

A mixture of methionine (A), glutamic acid (B) and lysine (C) is separated by paper electrophoresis in two  experiments, one at pH=1 and one at pH=12.  After the separation, the paper strips are treated with ninhydrin to reveal the location of amino acids (Purple spots). Which of the following correct match is of strips with appropriate pH?

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a

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b

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c

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d

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answer is A, B.

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Detailed Solution

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At pH1 (A) Methionine exist as +1 cation 
                        (B) Glutamic acid exist as +1  cation
                         (C)  Lysine exist as +2 cation
Electrophoretic mobility is directing proportional to magnitude of change   
At  pH12 Methionine exist as 1 anion  
                             Lysine exist as 1 anion
                    Glutamic acid exist as  2 anion

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