A mixture of serine (A), glutamic acid (B) and lysine (C) is separated by paper electrophoresis in two experiments one at pH ‘1’ and one at pH ‘12’. After the separation, the papers are treated with ninhydrin to reveal the location of amino acids (purple spots). Which of the following is correct match of strips with appropriate pH?

A- Neutral amino acid.

B- Acidic amino acid.

C- basic amino acid.

The main factor that effect electrophoretic mobility is magnitude of charge an ion. Greater the magnitude of charge greater is the electrophoretic mobility.

At pH = 1 A & B  posses +1 charge and C posses +2 charge .

So, these migrate towards cathode and ‘C’ has more electrophoretic mobility.

At pH=12 A & C possess ‘-1’ charge and ‘B’ possess ‘-2’ charge So, these migrate towards anode and ‘B’ has more electrophoretic mobility .