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Q.

A recombinant DNA molecule, created using a human gene, is inserted in a bacterial cell. But, the transformed cells did not produce the desired protein because

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a

there is a structural difference in all amino acids in bacteria and humans

b

human protein is formed, but degraded by bacterial enzymes

c

splicing mechanism for removing introns from protein coding RNAs is absent in bacteria

d

bacteria cannot recognise the codons on mRNA

answer is C.

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Detailed Solution

Human produce mRNA with introns. The introns are removed using splicing mechanism(spliceosome) before the translation process. In bacteria, the splicing mechanism is absent due to which the mRNA cannot be read for the desired protein.

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