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Q.

A student gets fingerprints on a cuvette before using it to determine the concentration of a coloured species using its known extinction coefficient. What is the effect on the absorbance and reported concentration?

Absorbance         Reported concentraion 

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a

increased                  too low

b

increased               too high

c

decreased                too low

d

decreased                           too high

answer is B.

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Detailed Solution

Absorbance is directly proportionate to the substance's concentration, according to the relationship between concentration and absorbance. The absorbance of a substance increases with its concentration. This is due to the fact that the quantity of molecules with which light interacts determines the amount of light that is absorbed.

Hence, option B is correct.

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A student gets fingerprints on a cuvette before using it to determine the concentration of a coloured species using its known extinction coefficient. What is the effect on the absorbance and reported concentration?Absorbance         Reported concentraion