(a) There are two different farm lands, one where Bt-cotton crop was cultivated and the other where non Bt-cotton crop (indigenous) was cultivated. Farmers responsible for this experimental cultivation were free to use the farming practices of their choice. During the cultivation period, the water requires for irrigation and at harvesting time, the crop productivity. Based on the data collected, a bar graph was plotted which is shown below.

Answer the following questions:
(i) Write your interpretation, with reason, on the basis of the three parameters plotted in the graph.
(ii) Which one out of the crops would you like to cultivate in your farm and why?
(iii) Which one out of these two crops would a farmer from Rajasthan like to cultivate and why?

OR

(b) Gene of interest/alien gene is introduced by a cloning vector into a host cell to bring about a desired phenotype expression in a host cell. The cloning vectors used are plasmid and bacteriophages. Biotechnologists in their labs, for desired results engineered specialised cloning vectors. One such vector is pBR322. Study the diagram carefully and answer the questions that follow.

(i) What do ‘EcoR I’, ‘BamH I’ and ‘Hind III’ represent? State their functions.
(ii) Identify the gene you would select for the role of a selectable marker in pBR322. Explain why.
(iii) Write the property/characteristic of plasmid and bacteriophage that makes them efficient cloning vectors.
(iv) Biotechnologists always insert ‘ori’ gene in their engineered cloning vector. Justify the statement.
(v) Will the experiment be successful if the alien DNA is ligated at Hind III restriction site? Give reason in support of your answer.

Question

(a) There are two different farm lands, one where Bt-cotton crop was cultivated and the other where non Bt-cotton crop (indigenous) was cultivated. Farmers responsible for this experimental cultivation were free to use the farming practices of their choice. During the cultivation period, the water requires for irrigation and at harvesting time, the crop productivity. Based on the data collected, a bar graph was plotted which is shown below.

Answer the following questions:
(i) Write your interpretation, with reason, on the basis of the three parameters plotted in the graph.
(ii) Which one out of the crops would you like to cultivate in your farm and why?
(iii) Which one out of these two crops would a farmer from Rajasthan like to cultivate and why?

OR

(b) Gene of interest/alien gene is introduced by a cloning vector into a host cell to bring about a desired phenotype expression in a host cell. The cloning vectors used are plasmid and bacteriophages. Biotechnologists in their labs, for desired results engineered specialised cloning vectors. One such vector is pBR322. Study the diagram carefully and answer the questions that follow.

(i) What do ‘EcoR I’, ‘BamH I’ and ‘Hind III’ represent? State their functions.
(ii) Identify the gene you would select for the role of a selectable marker in pBR322. Explain why.
(iii) Write the property/characteristic of plasmid and bacteriophage that makes them efficient cloning vectors.
(iv) Biotechnologists always insert ‘ori’ gene in their engineered cloning vector. Justify the statement.
(v) Will the experiment be successful if the alien DNA is ligated at Hind III restriction site? Give reason in support of your answer.

Infinity Learn · Accepted Answer

From the given graph, we conclude that Bt-crops have more productivity than those non-Bt-crops. Bt crops require less pesticide and more water for irrigation in comparison to non-Bt crops.

Bt crops give better outcomes than non-Bt as 40 Tonnes/Ha more productivity and requires 20 Litres/Ha less pesticides than non-Bt crops with minimal irrigation quantity of 60 Litres/Ha.

Farmer of Rajasthan should cultivate Bt-cotton crops as it is resistant to cotton boll worm that require fewer pesticide than non Bt-cotton-crops (indigenous), and productivity of crop per hectare also increases.

OR

‘EcoR I’, ‘BamH I’ and ‘Hind III’ are restriction endonucleases and their function is to cleave the foreign DNA at a specific site.

Selectable markers in pBR322 are ampicillin and tetracycline resistance genes. Selectable markers help in the identification of transformed cells from non-transformed cells.

Plasmid and bacteriophage can form high copy numbers efficiently. Plasmids are non-essential parts of bacteria and replicate independently and bacteriophages have better replication mechanisms and gene transfer.

In cloning vector, 'ori' gene helps in the initiation of DNA replication.

No, the experiment will not be successful as it will result in non-recombinant transformants.

Detailed Solution

Best Courses for You

Get Expert Academic Guidance – Connect with a Counselor Today!