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Q.

A transverse section of the stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained phloem?

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a

Orange

b

Pink

c

Red

d

Green

answer is B.

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Detailed Solution

The xylem absorbs red colour with safranin because the walls of the xylem cells contain lignin which takes up the red colour from the dye of safranin and phloem is coloured green with fast green as fast green stains the dense cytoplasm and cell walls present in the phloem.

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