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Q.

Arrange the following steps for making the bacterial cell competent to take up DNA, in chronological order.

I.    Incubation of cells with recombinant DNA on ice.

II.   Incubation of cells with recombinant DNA at 42 degree Celsius

III.   Treating cells with a specific concentration of a divalent such as calcium

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a

III, I, II, I

b

III, I, II

c

I, III, I, II

d

II, I, III

answer is A.

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Detailed Solution

In the method of rDNA technology, the bacterial cells must be made competent by treating with divalent cation like Ca. The bacteria along with rDNA must be kept on ice and later for brief period should be treated to 420C temperature and again be kept on ice.

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Arrange the following steps for making the bacterial cell competent to take up DNA, in chronological order.I.    Incubation of cells with recombinant DNA on ice.II.   Incubation of cells with recombinant DNA at 42 degree CelsiusIII.   Treating cells with a specific concentration of a divalent such as calcium