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Q.

 Arrange the following steps of rDNA technology in correct sequence.
I. Extraction of recombinant protein 
II. Culturing of recombinant host cells
III. Formation of rDNA  
IV. Isolation of desired DNA fragments
V. Isolation of DNA  
VI. Fragmentation of DNA
 

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a

V, VI, IV, III, II, I

b

VI, V, IV, III, II, I 

c

IV, V, VI, I, II, III

d

V, VI, IV, I, II, III

answer is A.

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Detailed Solution

The various steps in the rDNA technology in a sequence are Isolation of genomic DNA from Donor, cutting the DNA into fragments, separation of DNA fragments to isolate the desired DNA fragment or gene, Incorporating the gene or DNA fragment into vector to form rDNA, inserting rDNA into host cells, culturing the genetically modified organism to obtain recombinant protein and extracting and processing the recombinant protein from the bioreactors. 

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 Arrange the following steps of rDNA technology in correct sequence.I. Extraction of recombinant protein II. Culturing of recombinant host cellsIII. Formation of rDNA  IV. Isolation of desired DNA fragmentsV. Isolation of DNA  VI. Fragmentation of DNA