Arrange the following steps of rDNA technology in correct sequence.
I. Extraction of recombinant protein
II. Culturing of recombinant host cells
III. Formation of rDNA
IV. Isolation of desired DNA fragments
V. Isolation of DNA
VI. Fragmentation of DNA

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V, VI, IV, III, II, I

VI, V, IV, III, II, I

IV, V, VI, I, II, III

V, VI, IV, I, II, III

answer is A.

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Detailed Solution

The various steps in the rDNA technology in a sequence are Isolation of genomic DNA from Donor, cutting the DNA into fragments, separation of DNA fragments to isolate the desired DNA fragment or gene, Incorporating the gene or DNA fragment into vector to form rDNA, inserting rDNA into host cells, culturing the genetically modified organism to obtain recombinant protein and extracting and processing the recombinant protein from the bioreactors.

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