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Arrange the steps of isolation of DNA from the source cell.
(a) Precipitation of DNA with chilled ethanol.
(b) Treatment of DNA strands with protease and RNase.
(c) Treatment of DNA strands with restriction endonuclease.
(d) Isolation of DNA from bacterial cells by the treatment of lysozyme.

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(d), (a), (b), (c)

(b), (a), (d), (c)

(d), (a), (c), (b)

(d), (b), (a), (c)

answer is D.

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Detailed Solution

The sequential steps in the isolation of gene from the donor organism bacterium is

Treating the bacterial cells with lysozyme

Treating the protoplasm with protease and RNase

Precipitating the genomic DNA with chilled ethanol

Subjecting the DNA with particular Restriction endonuclease treatment.

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