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Q.

Assertion (A) : The fragments of DNA are separated based on their size by a process called gel electrophoresis

Reason (R) : DNA fragments separated in agarose gel will be seen when they are stained with ethidium bromide followed by exposing to U.V rays

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a

A is true but R is untrue

b

Both A and R are correct R explains A

c

Both A and R are correct but ‘R’ is not the correct explanation of  ‘A’

d

A is incorrect but R is correct

answer is A.

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Detailed Solution

Both A and R are correct but ‘R’ is not the correct explanation of  ‘A’

Assertion (A) is correct. Smaller DNA fragments move more easily through the gel, while larger fragments move more slowly. As a result, the DNA fragments become separated into distinct bands based on their size. Therefore, gel electrophoresis is a common laboratory technique used to separate DNA fragments based on their size. 

Reason (R) is also true. To visualize the separated DNA fragments in the agarose gel, a staining agent like ethidium bromide is commonly used. Ethidium bromide binds to DNA and becomes fluorescent when exposed to ultraviolet (UV) light. This

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Assertion (A) : The fragments of DNA are separated based on their size by a process called gel electrophoresisReason (R) : DNA fragments separated in agarose gel will be seen when they are stained with ethidium bromide followed by exposing to U.V rays