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Q.

At restriction site for the enzyme EcoR I, how many hydrogen bonds are broken after breakage of phosphodiester bonds?

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a

11

b

8

c

6

d

4

answer is A.

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Detailed Solution

By the action of EcoR I, the phosphodiester bonds present between G and A of GAATTC sequence of both the strands are broken at the restriction site and the over hangs have unpaired AATT because of breakage of hydrogen bonds. 

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