Based on the following information, answer the questions given below
RNA interference (RNAi), is an evolutionarily conserved mechanism triggered by double-stranded RNA (dsRNA), causes gene silencing in a sequence-specific manner . This technique will be used to develop pest resistant plants . Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference. ( RNAi) takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to the complementary dsRNA molecule that binds to and prevents translation of the mRNA. Nematode-specific genes were introduced into the host plant by using Agrobacterium vectors. DNA produced both sense and anti-sense RNA in the host cells . These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and silenced the specific mRNA of the nematode.
Nematode specific genes were introduced into the tobacco host plant using a vector
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a
Agrobacterium
b
Retrovirus
c
Bacteriophase
d
Cosmid
answer is A.
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Detailed Solution
Nematode-specific genes were introduced into the host plant using Agrobacterium vectors.
Based on the following information, answer the questions given belowRNA interference (RNAi), is an evolutionarily conserved mechanism triggered by double-stranded RNA (dsRNA), causes gene silencing in a sequence-specific manner . This technique will be used to develop pest resistant plants . Several nematodes parasitise a wide variety of plants and animals including human beings. A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. A novel strategy was adopted to prevent this infestation which was based on the process of RNA interference. ( RNAi) takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to the complementary dsRNA molecule that binds to and prevents translation of the mRNA. Nematode-specific genes were introduced into the host plant by using Agrobacterium vectors. DNA produced both sense and anti-sense RNA in the host cells . These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and silenced the specific mRNA of the nematode.Nematode specific genes were introduced into the tobacco host plant using a vector