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Which technique is used to detect AIDS?

Western blot and ELISA (enzyme-linked immunosorbent assay) are both laboratory techniques used to detect and measure specific proteins in a sample.

Western blot is a method used to detect and identify specific proteins in a complex mixture. It involves separating proteins by size using gel electrophoresis, transferring the separated proteins to a membrane, and then probing the membrane with a specific antibody that binds to the protein of interest. The presence of the protein is then detected by a secondary antibody that is labeled with a marker, such as a radioactive or fluorescent label.

ELISA, on the other hand, is a type of assay that uses antibodies to detect specific proteins in a sample. It involves coating a plate with an antigen (the protein of interest), adding the sample containing the protein, and then adding an antibody that binds to the protein. The presence of the protein is then detected by adding a secondary antibody that is linked to an enzyme and measuring the enzymatic activity with a substrate.

Both Western blot and ELISA are commonly used in biomedical research, and both methods have their own advantages and limitations. Western blot is more sensitive and specific but also more time-consuming and laborious, while ELISA is faster and easier but less sensitive. ELISA is also commonly used for diagnostic purposes, such as testing for HIV, Hepatitis B, and Lyme disease.