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During PCR,

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a

annealing is done after denaturation

b

Taq polymerase activity is seen prior to annealing

c

two sets of primers are used during melting for a gene amplification

d

very high temperature (94⁰C) is maintained during annealing.

answer is A.

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Detailed Solution

The sequence of events in PCR is denaturation of melting, annealing or attachment of primers to the DNA templates, extension of primers by the activity of Taq polymerase by using deoxyribonucleoside triphosphates. Annealing is done after deanaturation and prior to extension in PCR,

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