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Q.

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

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a

polysaccharides

b

RNA

c

histones

d

DNA

answer is C.

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Detailed Solution

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In recombinant DNA technology, during the purification of DNA, chilled ethanol is used for precipitation. The DNA is further isolated by spooling. Chilled ethanol promotes the formation of strong ionic bonds between
phosphates (PO32- of DNA and cations, like Na2+ and NH4 +, rendering it insoluble in the buffer.

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