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Q.

E.coli divides in 20 minutes. If  N15N15 containing E.coli is cultured in N14 medium then after 20 minutes

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a

all bacteria will have heavy DNA

b

50% bacteria will have hybrid DNA, 50% will have light DNA

c

all bacteria will have light DNA

d

all bacteria will have hybrid DNA

answer is C.

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Detailed Solution

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If E. coli containing N15N15 DNA is cultured in an N14 medium, and E. coli divides every 20 minutes, after 20 minutes of growth in the N14 medium, the distribution of DNA molecules within the bacterial population would be as follows:

Generation 1 (20 minutes): After one generation of growth (20 minutes), all the DNA in the population would become 14N15N (Hybrid DNA).

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