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Q.

Eli Lilly, an American company, prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E.coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating

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a

H-bonds

b

ionic bonds

c

peptide bonds 

d

disulphide bonds

answer is D.

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Detailed Solution

Chains A and B (of humulin) were produced separately, extracted and combined by creating disulphide bonds.

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