Explain the roles of the following with the help of an example each in recombinant DNA technology.
(a) Restriction enzymes
(b) Plasmids

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Detailed Solution

(a) Restriction enzymes or restriction endonucleases are essential tools for recombinant DNA technology that cut a DNA molecule at a specific site of four to six nucleotides. Example: EcoRI, HindIII.
(b) Plasmid is extrachromosomal self-replicating circular and double-stranded bacterial DNA. Plasmids act as a cloning vector in recombinant DNA technology. Example - pBR322.

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