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Q.

Following are various events involved in the sequencing of human DNA. Select the correct order.
a) Insertion into vectors.
b) Restriction digestion of DNA.
c) Amplification of DNA.
d) Isolation of DNA.
e) Sequencing.

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a

d, b, a, c, e.

b

b, d, a, c, e.

c

c, d, a, b, e.

d

d, a, c, b, e.

answer is A.

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Detailed Solution

For sequencing, the total DNA from a cell is isolated and converted into random fragments of relatively smaller sizes (recall DNA is a very long polymer, and there are technical limitations in sequencing very long pieces of DNA) and cloned in suitable host (YAC / BAC) using specialised vectors. The cloning resulted into amplification of each piece of DNA fragment so that it subsequently could be sequenced using automated sequencers.

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Following are various events involved in the sequencing of human DNA. Select the correct order.a) Insertion into vectors.b) Restriction digestion of DNA.c) Amplification of DNA.d) Isolation of DNA.e) Sequencing.