Formation of carbamino-haemoglobin occurs mainly at the

Carbamino-hemoglobin is a type of hemoglobin that forms when carbon dioxide (CO₂) binds to hemoglobin in the blood. The formation of carbamino-hemoglobin occurs mainly at the tissues in the body.

When oxygen-rich blood reaches the tissues, oxygen molecules are released from hemoglobin to be utilized by the cells for metabolic processes. As a result, the hemoglobin in these oxygen-depleted red blood cells becomes available to bind with other molecules, such as carbon dioxide.

At the tissue level, carbon dioxide is produced as a waste product of cellular metabolism. Some of this CO2 dissolves in the blood plasma, but a significant amount also diffuses into the red blood cells. Once inside the red blood cells, CO₂ reacts with water (H₂O) and forms carbonic acid (H₂CO₃) in the presence of an enzyme called carbonic anhydrase.