Give an account of amplification of gene of interest using PCR.

PCR Stands for polymerase chain reaction. It is a technique used for gene amplification in vitro.
Each cycle of PCR has three steps. They are :
i) Denaturation
ii) Primer annealing
iii) Extension of primers.

* High temperature induce denaturation of DNA.
* Two sets of primers, Taq polymerase enzyme and deoxyribonucleotides are added to denatured DNA.
* Taq polymerase extends the primery using the deoxyribonucleotides.
* As a result of repetition of this process many times, the segment of DNA can be amplified to approximately billion times.