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Q.

Given below are the steps during the cloning process of dolly the sheep. Arrange the steps in the correct order. 

  1. The nucleus was removed from the egg of the Scottish blackface ewe. 
  2. A cell was collected from the mammary gland of female Finn Dorsett sheep, and an egg was obtained from a Scottish blackface ewe.
  3. The egg with the transplanted nucleus was subjected to an electric shock to stimulate cell division and development.
  4. The nucleus of the cell from the Finn Dorsett sheep was inserted into the egg from which the nucleus was removed.
  5. The embryo developed into a lamb that was genetically identical to the donor Finn Dorsett sheep.
  6. The resulting embryo was implanted into the uterus of a surrogate mother, in this case, another Scottish blackface ewe.

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a

1 - 2 - 3 - 4 - 5 - 6

b

1 - 3 - 2 - 4 - 6 - 5

c

2 - 3 - 1 - 4 - 5 - 6

d

2 - 1 - 4 - 3 -  6 - 5

answer is A.

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Detailed Solution

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The cloning of Dolly the sheep was achieved through somatic cell nuclear transfer, a complex process that involved several steps. The correct order of these steps is as follows:

  1. A cell was collected from the mammary gland of a female Finn Dorsett sheep, and an egg was obtained from a Scottish blackface ewe.
  2. The nucleus was removed from the egg of the Scottish blackface ewe.
  3. The nucleus of the cell from the Finn Dorsett sheep was inserted into the enucleated egg.
  4. The egg with the transplanted nucleus was subjected to an electric shock to stimulate cell division and development.
  5. The resulting embryo was implanted into the uterus of a surrogate mother, in this case, another Scottish blackface ewe.
  6. The embryo developed into a lamb that was genetically identical to the donor Finn Dorsett sheep.

By arranging these steps in the correct order, scientists were able to successfully clone Dolly, the first mammal to be cloned from an adult somatic cell.

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