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Q.

Given below are two statements. One is labeled Assertion(A) and the other is labeled Reason(R).

Assertion (A): The recombinant will grow in ampicillin that contains medium but not in tetracycline containing medium, if the desirable gene is ligated at BamH I of pBR 322.
Reason (R): Non-recombinants with pBR 322 will grow in medium containing ampicillin and medium containing tetracycline.

In light of the above statements, choose the correct answer from the options given below.

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a

Both (A) and (R) are correct and (R) is the correct explanation of (A)

b

Both (A) and (R) are correct, but (R) is not the correct explanation of (A)

c

(A) is true but (R) is false

d

Both (A) and (R) are false.

answer is B.

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Detailed Solution

The recombinant will grow in the ampicillin medium but not in the tetracycline containing medium, because the tet gene has undergone insertional inactivation.

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