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Q.

Given below are two statements.
Statement-I:  Primers are used in PCR technique.

Statement-I: Primers provide 3' OH for the extension of DNA strand.

In light of the above statements, choose the correct answer from the options given below.

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a

Statement I is correct, but statement II is incorrect.

b

Both statement I and statement II are correct.

c

Both statement I and statement II are incorrect.

 

d

Statement  I is incorrect, but statement II is correct.

answer is A.

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Detailed Solution

Both Statement-I and Statement-II are correct.

Statement-I: Primers are used in the PCR (Polymerase Chain Reaction) technique. PCR is a molecular biology method used to amplify specific DNA sequences. Primers are short, single-stranded DNA sequences that are designed to be complementary to the target DNA sequences at the 3' end of each strand. They are used to initiate DNA replication during the PCR process.

Statement-II: Primers provide a 3' OH group for the extension of the DNA strand. In DNA replication, DNA polymerase enzymes can only add new nucleotides to an existing DNA strand in the 5' to 3' direction. Primers are designed to anneal (bind) to the target DNA at the specific sequence of interest, and their 3' end serves as the starting point for DNA synthesis. DNA polymerase then extends the primer by adding nucleotides in the 5' to 3' direction, creating a complementary copy of the DNA template strand. This extension process is a key step in PCR and various other DNA-related processes.

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