Given below are two statements.
Statement – I : Restriction endonucleases are used in rDNA technology

Statement – II : Restriction endonucleases cut the strand of DNA a little away from the center of the palindrome sites, resulting in fragments with sticky ends.

In light of the above statements, choose the correct answer from the options given below.

Both Statement I and Statement II are correct.

Statement I: Restriction endonucleases are used in rDNA (recombinant DNA) technology. These enzymes are essential tools in molecular biology and genetic engineering. They are used to cut DNA molecules at specific recognition sites, allowing scientists to manipulate DNA for various purposes, such as gene cloning, DNA fragment analysis, and the creation of recombinant DNA molecules.

Statement II: Restriction endonucleases cut the DNA strand a little away from the center of the palindrome sites, resulting in fragments with sticky ends. This is an accurate description of how restriction endonucleases work. These enzymes recognize specific DNA sequences, often palindromic sequences, and cleave the DNA strands at or near these recognition sites. The cleavage often leaves "sticky ends" with overhanging single-stranded regions, which can be used for the insertion of foreign DNA fragments into a plasmid or vector during molecular cloning. The complementary sticky ends can base-pair with each other, facilitating the formation of recombinant DNA molecules.