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Q.

Given diagram depicts the experiment of Meselson and Stahl. Identify the type of isotopic DNA formed after 40 minutes (A, B, C and D).

Question Image
 ABCD
114N DNA15N DNA14N DNA15N DNA
214N DNA15N DNA14N DNA14N DNA
314N DNA14N DNA15N DNA15N DNA
414N DNA15N DNA15N DNA15N DNA

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a

A-14N-DNA, B-15N-DNA, c-14N-DNA, D-155N-DNA

b

A-14N-DNA, B-15N-DNA, C-14N-DNA, D-14N-DNA

c

A-14N-DNA, B-I144N-DNA, C-15N-DNA, D-15N-DNA

d

A-14N-DNA, B-15N-DNA, C-15N-DNA, D-15N-DNR

answer is B.

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Detailed Solution

In Meselson and Stahl experiment after 40 minutes , there will be formation of 50% hybrid DNA and 50% light DNA . here in given diagram A-DNA and D-DNA  are light DNA ( both the strands are with 14N),B-DNA and C-DNA are hybrid DNA (one strand is with 14N and another strand is with 15N).As D-DNA is light DNA ,there is no 15N strand in D-NA .

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