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Homozygous pure lines in cattle can be obtained by mating

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mating of related individuals of same breed

mating of individuals of different species

mating of unrelated individuals of same breed

mating of individuals of different breed

answer is D.

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Detailed Solution

Pure lines are obtained by inbreeding. Mating of individuals which are related by descent at least for 4-6 generations is referred to as inbreeding.

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