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Q.

How many statements are incorrect ?
(i) Cell walls are digested by treating the bacterial cells/ plant or fungal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).
(ii) Purified DNA is precipitated by addition of chilled ethanol.
(iii) Polymerase Chain Reaction (PCR) require thermostable DNA ploymerase which remains
active during the high temperature and induced denaturation of double stranded DNA
(iv) If any protein encoding gene is expressed in heterologous host, it is called a recombinant
protein.

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a

III statement

b

IV statement

c

I statement

d

None of these

answer is A.

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Detailed Solution

Statement iii is incorrect.
PCR require thermostable DNA polymerase. which remains active at high temparature they induce polymerisation not denaturation of ds DNA.

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