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Q.

Identify the A, B, C respectively in the following diagram 

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a

Site action on ligase, Clipping end, rDNA 

b

Site of action of EcoRI, Clipping end and rDNA

c

Palindromic sequence of RNA, Ligation, rDNA 

d

Site action of EcoRI, Blunt end, hybrid DNA  

answer is A.

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Detailed Solution

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A is site of action of EcoRI in between G and A of GAATTC palindromic sequence on both the strands of DNA. B is the clipping end of the fragment with overhang as AATT and C is the rDNA formed upon ligase action between two DNA fragments of different origin.

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Identify the A, B, C respectively in the following diagram