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Q.

Identify the correct statement about ‘sticky ends’.
(a) Single stranded extensions of DNA ends cut by some restriction endonucleases
(b) Hydrogen bonds are absent in sticky ends
(c) Can form hydrogen bonds with another sticky end having complementary bases

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a

a only

b

b only

c

c only

d

a, b and c

answer is D.

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Detailed Solution

Sticky ends are single-stranded overhangs that result from the cleavage of DNA by certain restriction endonucleases.

Hydrogen bonds are absent in the single-stranded regions of sticky ends. Sticky ends are created when some restriction endonucleases cut DNA, leaving single-stranded overhangs. 

Sticky ends are single-stranded extensions of DNA ends that are generated when some restriction endonucleases (restriction enzymes) cleave DNA at specific recognition sites. These single-stranded overhangs can base-pair with complementary sequences, allowing for the specific and precise joining of DNA fragments during processes like DNA cloning and recombinant DNA technology. The formation of hydrogen bonds between complementary bases in the sticky ends is a crucial step in these processes.

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