Q.

Identify the incorrect statement. 

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a

Insertional inactivation of β-galactosidase gene leads to blue colour colonies.

b

In insertional inactivation, the rDNA in inserted within the coding sequence of an enzyme β-galactosidase.

c

Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.

d

Insertional inactivation of enzyme β-galactosidase leads to colourless colonies.

answer is A.

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Detailed Solution

Insertional inactivation is a technique that is used in recombinant DNA technology. During insertional inactivation of β galactosidase gene no blue color colonies develop as beta galactosidase is not produced due to deactivation of the lacZ gene.

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