Q.

If alien DNA is inserted within the coding sequence of enzyme β-galactosidase, which of the following will occur in case of non-recombinants?

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a

Enzyme β-galactosidase will not form

b

Chromogenic substrate gives blue colour

c

Insertional inactivation of gene coding for β-galactosidase

d

No colour is produced by the colonies

answer is C.

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Detailed Solution

In non-recombinants, alien DNA is not inserted in the sequence of enzyme β-galactosidase. So, the enzyme will produce blue colour on addition of chromogenic substrate.

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