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Q.

If alien DNA is introduced using Sal I in pBR322 then

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a

βgalactosidase help in identifying recombinants

b

Tetracycline help in identifying recombinants

c

Ampicillin help in identifying recombinants

d

White colony help in identifying recombinants

answer is A.

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Detailed Solution

If alien DNA is introduced using Sal I, vector looses tetracycline resistance  because Sal I restriction site is present in tetracyclin region. So here in  pBR322  tetracycline help in identifying recombinants .

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If alien DNA is introduced using Sal I in pBR322 then