If Cla I enzyme is used for inserting a gene for $β$ galactosidase production and the recombinant pBR322 is inserted in an E.coli strain, then

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It will be able to confer ampicillin resistance only to host cell as well as produce $β -$ galactosidase

It will be able to confer both ampicillin and tetracycline resistance to host cell as well as produce $β -$ galactosidase

It will lose resistance to tetracycline and does not produce $β -$ galactosidase

It will not produce $β -$ galactosidase

answer is B.

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Detailed Solution

ClaI site is located neither within the amp^R site nor within the tet^R site.So if Cla I enzyme is used for inserting a gene for $β$ galactosidase , the recombinant E.coli strain,exhibits resistance for ampicillin and tetracyclline.It also expresses the gene $β$ galactosidase.

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