If E. coli were allowed to grow in the culture medium for 80 minutes by Mathew Meselson and Franklin Stahl in their experiments, the proportion of light and hybrid density DNA molecule would be

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87.5% of light density DNA and 12.5% of hybrid density DNA

75% of light density DNA and 25% of hybrid density DNA

50 % of light density DNA and 50% of hybrid density DNA

12.5% of light density DNA and 87.5% of hybrid density DNA.

answer is A.

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Detailed Solution

Meselson- Stahl experiment showed the semiconservative nature of DNA. E.coli growing in presence of ammonium chloride with heavy isotope of nitrogen (15 N) were transferred to 14N normal isotope containing medium. 1 distinct band of 15N-14N was obtained after 20 mins. 2nd generation have half 15N-14N and other 14N-14N DNA. In this manner after 80 mins large fraction of extracted DNA is 14N-14N.

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