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Q.

If we ligate a foreign, DNA at the Bam HI site of tetracycline resistance gene in pBR322 the recombinant plasmid will: 

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a

Show ampicillin resistance only 

b

Show tetracycline resistance 

c

Will grow well on tetracycline containing medium

d

Will not grow on ampicillin containing medium

answer is A.

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Detailed Solution

If a gene is inserted at Bam H I site of pBR322, the selectable marker tetracyclin resistance gene is inactivated and the recombinants show resistance to only ampicillin but not to tetracyclin.

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