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Q.

If we use enzyme PvuI during formation of recombinent DNA by using PBR322 as vector then the antibiotic resistance gene used as selectable marker to differentiate between transformant and non transformant is

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a

ampRgene

b

tetRgene

c

ampR or tetRgene

d

It is not possible to differentiate

answer is B.

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Detailed Solution

If the gene is inserted at PvuI, the gene for ampR is inactivated and only tetR is used as marker to select tansformants from non-transformants.

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